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. 2020 Oct 31;8(11):467. doi: 10.3390/biomedicines8110467

Figure 2.

Figure 2

The effect of relaxin (RLX)-2 on endometriotic stromal cells (ESCs). ESCs were incubated with RLX-2 for 24 h, and the concentration of cAMP in conditioned media was measured by a specific assay kit. Representative data from three different experiments were shown as the mean ± SEM *: p < 0.05 compared to the control (a). After 8 h stimulation with recombinant-RLX-2 (rRLX-2, 100 ng/mL), real-time RT-PCR was performed to measure the mRNA expression levels of collagen-I, plasminogen activator inhibitor-1 (PAI-1), connective tissue growth factor (CTGF), and α-smooth muscle actin (α-SMA) in the control and RLX-2-treated group. Data were normalized by GAPDH mRNA levels to show the relative abundance. Representative data from three different experiments were shown as the mean ± SEM relative to an adjusted value of 1.0 for the mean value of the control (b). ESCs were incubated with RLX-2 for 24 h, and the concentration of IL-8 (pg/mL) in conditioned media was measured by specific assay kits (c). *: p < 0.05.