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. 2020 Nov 26;10:20708. doi: 10.1038/s41598-020-76838-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of calcification of aortas. (a) Representative photomicrographs of the aorta with von Kossa stain (magnification, ×40). (b) Representavive photomicrographs of the aorta with von Kossa stain (magnification, ×200). (c) The percentage of von Kossa-positive mineralized area of aortas. (d) Calcium contents of aortas. DM, diabetes mellitus; CKD, chronic kidney disease; INS, insulin; APO, apocynin; Ca, calcium. ^aVersus Control group, P < 0.05, ^bversus DM group, P < 0.05, ^cversus CKD group, P < 0.05, ^dversus CKD + DM, P < 0.05.