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. 2020 Oct 31;8(11):465. doi: 10.3390/biomedicines8110465

Figure 1.

Figure 1

Effects of family with sequence similarity 188 member B (FAM188B) knockdown on proliferation and cell cycle in lung cancer cell lines. (A) Equal amount of cellular proteins from Beas-B2, a normal lung cell line, and A549 and H1299 lung cancer cell lines was processed for immunoblot analysis using indicated antibodies. GAPDH was used as a loading control. (B–E) A549 and H1299 cells were transfected either with non-specific control siRNA (NC siRNA) or siRNA specific for FAM188B (siFAM188B) at indicated times. Cells were then either processed for immunoblot analysis using indicated antibodies (B), cell growth analysis using Incucyte™ (C), cell-cycle analysis by flow cytometry (D), or apoptosis assay by flow cytometry of annexin-V/PI stained cells (E). Annexin-V-positive populations were considered as apoptotic populations. Scale bar; 100 μm. These experiments were performed three times with comparable results.