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. 2020 Nov 26;10:20726. doi: 10.1038/s41598-020-77828-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative TUNEL assay to visualize the apoptotic rate (red signal; arrows indicate positive cells) of tracheal sections after all treatments. (A) Vehicle control sample, (B) GNP-HC and (C) GNP-HCIm. (D) Graphical quantification of TUNEL staining. Graph was done by Graphpad Prism 6.0; (https://www.graphpad.com/scientific-software/prism/). Asterisks = lumen of trachea. 20 × magnification is shown. Data were represented as mean (N = 8 for each group) ± SEM and analyzed by one-way ANOVA followed by a Bonferroni post-hoc test for multiple comparisons. ###p < 0.05. Scale bar = 100 µm.