Figure 2.

Relative expression of MEG3 in serum of patients with COPD patients and CSE-induced 16HBE cells. (A) Expression of MEG3 was determined using RTq-PCR in the serum of patients with COPD or healthy controls. (B) MEG3 level was determined using the RT-qPCR in16HBE cells and 16HBE cells treated with CSE (2%, 24 h). *P<0.05 vs. respective control. MEG3, maternally expressed gene 3; COPD, chronic obstructive pulmonary disease; CSE, cigarette smoke extract; NC, negative control; Con, control; RT-q, reverse transcription-quantitative.