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. 2020 Oct 28;12(11):1221. doi: 10.3390/v12111221

Figure 3.

Figure 3

TNF drives ∆M36-dependent apoptosis in endothelial cells and fibroblasts. (A). Relative viability of WT BMDM and SVEC4-10 cells infected with K181 or ∆M36 (MOI = 10) for 24 h in the absence or presence of murine TNF (25 ng/mL). TNF was added to medium 1 hpi. Graph reflects data pooled from three independent experiments, each containing 3 replicates. (B). IB showing the appearance of Cl-C8 and Cl-C3 with loading control β-actin 14 hpi in WT or SVEC4-10 cells under indicated conditions. Data are representative of 2 independent experiments. (C–E). Relative viability of SVEC4-10 treated with no cytokine, murine IFNβ or IFNγ (100 ng/mL each), (C), MEFs of indicated genotypes treated with murine TNF (25 ng/mL) (D) or HF treated with human TNF (100 ng/mL) (E) and infected with K181 or ∆M36 (MOI = 10) at 24 hpi. All cytokines were added to the medium at 1 hpi with virus. C-E reflect data pooled from two independent experiments, each containing 3 replicates. Lines above bars indicate the two groups compared for significance values using paired, parametric Student’s t-test where * is <0.05, ** is <0.01, and n.s. represents nonsignificant.