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. 2020 Nov 26;10:20715. doi: 10.1038/s41598-020-77817-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles of Foxl1 homeolog mRNAs in the small intestine during natural and T3-induced metamorphosis. Total RNA was prepared from the intestine of X. laevis tadpoles at indicated developmental stages (a,c) or Nieuwkoop and Faber (NF) stage 54-tadpoles after 10 nM T3 treatment (b,d) and was analyzed by qRT-PCR. Levels of Foxl1.L (a,b) and Foxl1.S mRNAs (c,d) are shown relative to those of ribosomal protein L8 (rpL8.S) mRNA, with the values at stage 54 or T3 for 0 day set to 1^41–43. Error bars represent the SEM (n = 4). The values were analyzed by ANOVA followed by Scheffe’s post hoc test. Different letters indicate significant differences among the indicated stages or days after T3 treatment (P < 0.05).