Figure 3. Changes in gene expression in lacrimal glands from male NOD mice at and after 6 weeks of age as revealed by transcriptome analysis.
A, experimental design of transcriptome analysis. Total RNA was extracted from lacrimal glands from male BALB/c and NOD mice at 4, 6 and 10 weeks of age, and then RNA‐seq was performed. The experiment was conducted with three animals in each group. B, multidimensional scaling plot, showing similarity of the gene expression patterns. C, heat map of one‐way hierarchical clustering using Z‐score for normalized values (log2‐based). This graphically represents the similarity of expression patterns between samples and genes.