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. 2020 Oct 31;9(11):2387. doi: 10.3390/cells9112387

Table 1.

Primers sequences used for SYBR Green assays.

Genes Primer Sequences Tm T° qPCR Amplicons Genes Number
GFAP F AGAGGAGTGGTATCGGTCTAAGTTT 56.9 °C 60 °C 167 pb NM_001131020.1
R GCCGCTCTAGGGACTCGTTC 59.8 °C NM_010277.3
Cx43 (mGja1) F AGAGCCCGAACTCTCCTTT 55.7 °C 60 °C 247 pb NM_010288.3
R GTTCATCACCCCAAGCTGAC 56.1 °C
Cx 30 (mGjb6) F CTGTCCCCGATTCCATTCCC 57.9 °C 60 °C 292 pb NM_001010937.2
R CATCGTGCAGGCTTATTCTGAG 55.8 °C
Cx 26 (mGjb2) F GCGACCCATTTCGGACCAA 58.2 °C 60 °C 159 pb NM_008125.3
R GAGTGTGCCCCAATCCATCT 57.3 °C
mPanx1 F CAGGCTGCCTTTGTGGATTC 56.7 °C 60 °C 145 pb NM_019482.2
R CGGGCAGGTACAGGAGTATG 57.2 °C
mGAPDH F TTCTCAAGCTCATTTCCTGGTATG 54.9 °C 60 °C 143 pb NM_008084.3
R GGATAGGGCCTCTCTTGCTCA 58.4 °C