Table 1.
Genes | Primer Sequences | Tm | T° qPCR | Amplicons | Genes Number | |
GFAP | F | AGAGGAGTGGTATCGGTCTAAGTTT | 56.9 °C | 60 °C | 167 pb | NM_001131020.1 |
R | GCCGCTCTAGGGACTCGTTC | 59.8 °C | NM_010277.3 | |||
Cx43 (mGja1) | F | AGAGCCCGAACTCTCCTTT | 55.7 °C | 60 °C | 247 pb | NM_010288.3 |
R | GTTCATCACCCCAAGCTGAC | 56.1 °C | ||||
Cx 30 (mGjb6) | F | CTGTCCCCGATTCCATTCCC | 57.9 °C | 60 °C | 292 pb | NM_001010937.2 |
R | CATCGTGCAGGCTTATTCTGAG | 55.8 °C | ||||
Cx 26 (mGjb2) | F | GCGACCCATTTCGGACCAA | 58.2 °C | 60 °C | 159 pb | NM_008125.3 |
R | GAGTGTGCCCCAATCCATCT | 57.3 °C | ||||
mPanx1 | F | CAGGCTGCCTTTGTGGATTC | 56.7 °C | 60 °C | 145 pb | NM_019482.2 |
R | CGGGCAGGTACAGGAGTATG | 57.2 °C | ||||
mGAPDH | F | TTCTCAAGCTCATTTCCTGGTATG | 54.9 °C | 60 °C | 143 pb | NM_008084.3 |
R | GGATAGGGCCTCTCTTGCTCA | 58.4 °C |