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. 2020 Nov 12;8:593685. doi: 10.3389/fcell.2020.593685

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Copyright © 2020 Pin, Huanting, Chengzhan, Xinjuan, Yugong, Wei, Shifang, Zhaojian, Kun, Weicheng, Yingying, Yongming and Yanan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PDCD4 regulates production of IL-5, CCL-5, VEGF, and CXCL10. (A) Western blot analysis of nuclear and cytosolic fractions of indicated cells. Histone 3 and tubulin are used as nuclear protein marker and loading control, respectively. (B) Luciferase reporter assay showing NF-κB transcriptional activity in cells over-expressing PDCD4. (C) Cytokine assay indicating levels of cytokines and chemokines in conditional media collected from cells over-expressing PDCD4. (D) Real-time PCR analysis depicting mRNA fold changes of the indicated genes in the cytokine assay. **P < 0.01.