Figure 3.

Pf4 phage variant infection leads to altered biofilm architecture and cell morphology. Side, top and three-dimensional views of 24 h-old biofilm of H103 untreated and treated with Pf4* phage grown under static (A) and dynamic conditions (B,C) observed by confocal laser scanning microscopy (CLSM). Cells were stained with SYTO9 green fluorescent dye. Values represent COMSTAT2 image analyses. Results were given ± SEM of at least three independent experiments. (C) Focus on microcolonies of the Pf4*-T biofilm grown in dynamic conditions. Each experiment was assayed at least four times independently.