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. 2020 Oct 30;8(11):1700. doi: 10.3390/microorganisms8111700

Figure 4.

Figure 4

bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) quantification and matrix-encoding gene expression increase after Pf4 phage variant treatment. (A). Relative mRNA expression levels of pelB, algD, pslB, amrZ, fleQ, pilA and cdrA in H103 (green bars) and Pf4*-T (violet bars) conditions as determined by RT-qPCR experiments. (B). Relative c-di-GMP level measured by LC-MS/MS in H103 (green bar) and Pf4*-T conditions. Each experiment was assayed at least four times independently. Statistics were achieved by paired (two samples) two-tailed t-test. * p < 0.05; ** p < 0.01; **** p < 0.0001.