Maternal HF diet alters offspring hypothalamic tissue expression of AMPK, pAMPK and bHLH factors. (A) Hes1, Mash1, Ngn3, and (B) AMPK and pAMPK protein expression in hypothalami of 1-day-old males, and ARC of 12-month-old males from control (■) and HF (□) groups. Fold change (mean ± SE) of n = 6 males from six litters per group (1 male/litter). * p < 0.01 vs control. Abbreviations: HF (high fat); AMPK (5’ AMP-activated protein kinase); pAMPK (phosphorylated 5’ AMP-activated protein kinase); Hes1 (Hairy and Enhancer of Split 1); Mash1 (Achaete-scute complex homolog-1); Ngn3 (neurogenin 3); GAPDH (Glyceraldehyde 3-phosphate dehydrogenase).