Figure 3.

A769662, but not metformin, reverses the NaIO₃-induced mitochondrial oxygen consumption rate (OCR) decrease. Metformin (6 mM) (A) or A769662 (30 μM) (C) were pretreated 26 min prior to NaIO₃ injection through Port A. Then cells were subsequently treated with oligomycin, FCCP and antimycin A/rotenone. Intracellular OCR was measured by seahorse XF24 analyzer. (B,D) Resting OCR, ATP turnover and respiratory capacity were analyzed and the data were mean ± SEM from three independent experiments. * p < 0.05, indicating the significant effects of NaIO₃. ^# p < 0.05, indicating the inhibitory effects of A769662 on the action of NaIO₃.