Table 3.
Candidate insecticidal protein genes detected in the KhF strain.
| Gene Identity | Length (aa) | Scaffold | Closest Homolog | Similarity (%) | Coverage (%) | E-Value | SignalIP Server |
|---|---|---|---|---|---|---|---|
| peg5520 1 | 867 | 73 | WP_098163495.1|hypothetical protein N | 99.54 | 100 | 0.0 | Yes |
| AEB52299.1|Vip4Aa1 C | 35.81 | 57.83 | 6.0−93 | ||||
| peg5936 1 | 1223 | 81 | WP_153593625.1|ETX/Mtx2 family from Bt N | 67.67 | 100 | 0.0 | No |
| AGC08395.1|Vip1Ad1C | 26.9 | 60.60 | 1.0−21 | ||||
| peg309 | 333 | 114 | WP_088083339| hypothetical protein N | 97 | 100 | 0.0 | Yes |
| ADK79125.1|Vip1Aa1 C | 27.41 | 59.26 | 4.0−12 | ||||
| peg2096 | 566 | 31 | WP_000734347.1|hypothetical protein N | 100 | 100 | 0.0 | Yes |
| AGC08395.1|Vip1Ad1 C | 25.62 | 58.68 | 5.0−11 | ||||
| peg3627 | 739 | 51 | WP_000811547| hypothetical protein N | 100 | 100 | 0.0 | Yes |
| AAR40886.1|Vip1Ba1 C | 27.97 | 58.05 | 2.0−12 | ||||
| peg4213 | 347 | 59 | WP_098475832| RICIN domain-containing protein N | 80.35 | 100 | 0.0 | Yes |
| CAH56541.1|Cry49Aa1 C | 23.98 | 67.54 | 7.0−25 | ||||
| peg5521 | 184 | 73 | PHE82359.1| hypothetical protein N | 97.81 | 100 | 5.0−124 | No |
| CAI40768.1|Vip2Ad1 C | 27.88 | 61.21 | 9.0−13 | ||||
| peg5522 | 502 | 73 | WP_098163497.1|hypothetical protein N | 99 | 100 | 0 | Yes |
| AAO86513.1|Vip2Ac1 C | 28.64 | 60.56 | 8.0−14 | ||||
| peg5937 | 216 | 81 | WP_153593624.1|hypothetical protein from Bt N | 89.39 | 92 | 1.0−127 | No |
| - 2 | - | - | - |
N NCBI database; Blastx search results using the Non Redundant protein database of the NCBI. C Customized database; Blastx search results using a customized protein database performed using the Bt toxin nomenclature database. 1 The genes peg5520 and peg5936 have been predicted using the tool Btoxinscanner and Blastx. 2 The coding sequence peg5937 was not detected in the blast search against the customized Bt toxins database. - Not applicable.