Table 6.
Identification of the candidate insecticidal proteins in the KhF strain by LC-MS/MS analysis.
| Gene Identity | Protein Molecular Mass (kDa) | Unused ProtScore | Sequence Coverage (%) | Matched Peptides | Hi3 (%) 2 | Shared Peptides 3 | Unique Peptides 4 |
|---|---|---|---|---|---|---|---|
| peg5936 | 134.32 1 | 188.93 | 92.96 | 171 | 79.15 | 23 | 23 |
| peg5937 | 24.23 1 | 28.39 | 98.60 | 20 | 4.05 | 6 | 2 |
1 The size of the Peg5936 and Peg5937 were obtained from protein sequences derived and predicted by the Glimmer v2 software in the Rast server. 2 Hi3 is a quantitative proteomics technique that estimates the quantity of a protein by intensifying the LC-MS data of the tryptic peptides of a protein of interest in the mixture [35,36]. 3 Number of peptides with a contribution value higher than 2 that are present in two or more proteins of the described Cry toxins. 4 Number of peptides with a contribution value higher than 2 that are present only once in one protein of the described Cry toxins.