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A: IFNG expression in equine penile tumor samples. B: IL2 expression in equine penile tumor samples. Data are expressed as 2−ΔΔCq where ΔCq = Cq (target gene) − Cq (reference gene); values are the mean of three test replicates ± 1 standard deviation and ΔΔCq = ΔCq (samples TGFB+) − ΔCq (samples TGFB−). Negative samples were given a Cq 48 fictitious value. * p < 0.05.
