Table 1.
Antibodies and protocols used for the characterization of the immune infiltrate in equine penile squamous cell carcinomas (epSCCs).
| Antibody | Source | Working Dilution; Incubation | Antigen Retrieval | Reaction Pattern |
|---|---|---|---|---|
| CD3 | Rabbit polyclonal, Dako | 1:200; 1h RT | HIER, ph 9.0, TRIS-Edta | Membrane |
| CD20 | Rabbit polyclonal, Dako | 1:300; 1h RT | No AR | Membrane |
| FOXP3 | Rat monoclonal, eBioscience | 1:50; ON 4 °C | HIER, ph 9.0, TRIS-Edta | Nuclear |
| MUM1 | Mouse monoclonal, Dako | 1:200; 2h RT | HIER, ph 9.0, TRIS-Edta | Nuclear and cytoplasmic |
| IBA1 | Goat polyclonal, LSBio | 1:50; 2h RT | HIER, ph 9.0, TRIS-Edta | Cytoplasmic |
| MAC387 | Mouse monoclonal, Dako | 1:100; 2h RT | HIER, ph 9.0, TRIS-Edta | Cytoplasmic |
| Ki-67 | Mouse monoclonal, Dako | 1:50; 2h RT | HIER, ph 9.0, TRIS-Edta | Nuclear |