Table 6.
Histological diagnosis: SCC: squamous cell carcinoma; CIS: carcinoma in situ; P: papilloma. RT-PCR data for B2M are expressed as + (amplified) or – (not amplified); Indication of the viral load was give indicating the Cq at which the positivity for L1 was detected: − (>48 Cq), + (32–37 Cq), ++ (26–31 Cq), +++ (20–25 Cq), and ++++ (14–19 Cq). All samples were classified into EcPV2 high when viral load was +++ or ++++ and into EcPV2 low when viral load was ++ or +. RT-qPCR data are expressed as mean Cq ± 1-standard deviation of three replicates. Negative sample are given >48 Cq.
| ID | Histological Diagnosis | DNA | cDNA | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| B2M | L1 | Viral Load | L1 | B2M | IL5 | IL8 | IL10 | IL12/p35 | IL12/p40 | ||
| 1 | SCC | + | +++ | High | 33.5 ± 0.2 | 33.0 ± 0.2 | 37.4 ± 0.5 | 35.8 ± 1.5 | >48 | >48 | >48 |
| 2 | SCC | + | + | Low | >48 | 31.2 ± 0.1 | >48 | 35.4 ± 0.3 | >48 | 36.7 ± 1.5 | 37.2 ± 0.7 |
| 3 | CIS | + | +++ | High | 34.3 ± 0.3 | 33.2 ± 0.3 | >48 | 31.2 ± 1.6 | >48 | >48 | 35.4 ± 0.2 |
| 4 | SCC | + | ++++ | High | 35.1 ± 0.6 | 35.5 ± 0.4 | >48 | >48 | 37.8±0.9 | >48 | 36.5 ± 0.7 |
| 5 | P | + | + | Low | >48 | 32.5 ± 0.3 | 39.8 ± 2.3 | >48 | >48 | 35.0 ± 1.2 | 36.8 ± 0.1 |
| 6 | SCC | + | + | Low | >48 | 33.4 ± 1.0 | >48 | >48 | >48 | 45.0 ± 2.1 | 42.3 ± 7.7 |
| 7 | SCC | + | ++++ | High | 35.9 ± 0.7 | 34.5 ± 0.3 | >48 | >48 | >48 | >48 | 36.8 ± 0.1 |
| 8 | SCC | + | ++++ | High | 32.3 ± 0.3 | 33.0 ± 0.4 | >48 | 34.2 ± 0.2 | >48 | >48 | 36.9 ± 0.1 |
| 9 | SCC | + | ++++ | High | 34.9 ± 0.9 | 33.0 ± 0.5 | >48 | 36.0 ± 0.5 | >48 | >48 | 35.9 ± 0.2 |
| 10 | SCC | + | + | Low | >48 | 37.9 ± 0.9 | >48 | 34.9 ± 0.7 | >48 | 41.2 ± 2.5 | 36.5 ± 0.1 |
| 11 | SCC | + | ++++ | High | 33.0 ± 0.3 | 34.9 ± 0.8 | >48 | 35.9 ± 0.6 | >48 | >48 | 36.5 ± 0.5 |
| 12 | SCC | + | + | Low | >48 | 34.5 ± 0.3 | >48 | 37.2 ± 0.7 | >48 | 45.0 ± 3.4 | 37.6 ± 1.3 |
| 13 | SCC | + | ++ | Low | 36.6 ± 0.1 | 34.1 ± 1.3 | >48 | 36.7 ± 0.8 | >48 | >48 | >48 |
| 14 | SCC | + | ++++ | High | 31.3 ± 2.7 | 33.6 ± 0.5 | >48 | 36.7 ± 0.3 | >48 | >48 | 36.7 ± 0.6 |
| 15 | SCC | + | ++++ | High | 35.6 ± 0.1 | 34.2 ± 0.2 | >48 | 34.2 ± 0.1 | 39.7±4.6 | >48 | >48 |
| 16 | SCC | + | ++ | Low | 37.8 ± 1.5 | 27.9 ± 0.1 | 35.0 ± 1.1 | 26.1 ± 0.1 | 35.5 ± 1.7 | 38.2 ± 3.3 | 34.8 ± 0.1 |
| 17 | SCC | + | - | - | ND | 29.4 ± 0.2 | 42.6 ± 6.7 | 33.0 ± 0.7 | 36.5 ± 0.9 | 36.5 ± 1.7 | 35.9 ± 0.6 |
| 18 | CIS | + | +++ | High | 31.3 ± 2.7 | 32.6 ± 0.3 | 37.5 ± 0.9 | 30.5 ± 0.2 | >48 | >48 | 36.1 ± 0.6 |
| 19 | SCC | + | +++ | High | 35.3 ± 0.1 | 31.9 ± 0.5 | >48 | 33.4 ± 0.5 | 35.3 ± 1.5 | >48 | 36.0 ± 0.1 |
| 20 | SCC | + | - | - | ND | 32.1 ± 0.9 | >48 | 36.5 ± 0.7 | >48 | 45.6 ± 2.3 | 35.5 ± 0.3 |