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. 2020 Nov 12;11:561002. doi: 10.3389/fmicb.2020.561002

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Copyright © 2020 Wang, Zhang, Zhang, Zhang, Fu, Li, Wang, Qiu, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analyses of live/dead bacteria in E. coli ATCC 25922 biofilms: (A) Confocal laser scanning microscopy (CLSM) images of C. camphora essential oil (CCEO) at 0 μL/mL for 6, 12 and 24 h; (B) CLSM images of CCEO at 2 μL/mL for 6, 12 and 24 h; Green, live bacteria, red, dead bacteria; Images from three independent replicates with 20 μm bars are representative; (C) Biomass of live cells in bilfilm; (D) Ratio between dead and live cells in biofilm. A sample of 0 μL/mL CCEO was used as a control. Data represents the mean ± SD of three independent experiments, ** indicates p < 0.01 compared with the control at the same time.