Figure 1.

Schematic diagram for Agrobacterium-mediated Chinese cabbage transformation and generation of transgenic plants carrying both epithelial cell adhesion molecule–immunoglobulin M fragment crystallizable (EpCAM–IgM Fc) and Joining (J)-chain K. (A) Plant expression vectors to express EpCAM–IgM Fc and J-chain K for Agrobacterium-mediated transformation. The expected quaternary structure of EpCAM–IgM Fc in T₀ transgenic plants carrying the transgene encoding EpCAM–IgM Fc is hexameric. The expected protein structure of the J-chain K in T₀ transgenic plants carrying the transgene encoding J-chain K is hook-like. (B) Generation of T₀ transgenic plants carrying EpCAM–IgM Fc and J-chain K obtained from Agrobacterium-mediated transformation. (C) T₁ transgenic plants with EpCAM–IgM Fc and J-chain K obtained from self-crossing of T₀ transgenic plants with EpCAM–IgM Fc and J-chain K. Cross-fertilization between T₁ transgenic plants carrying EpCAM–IgM Fc and J-chain K transgenes after selfing of T₀ plant generation. (D) F₁ transgenic plants carrying both transgenes encoding EpCAM–IgM Fc and J-chain K obtained from crossing between T₁ transgenic plants carrying transgenes encoding EpCAM–IgM Fc and T₁ transgenic plants carrying transgenes encoding J-chain K. The expected quaternary structure of EpCAM–IgM Fc × J-chain K in transgenic plant F₁ is pentameric.