Figure 8.

Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in representative F₁ transgenic lines. (A) F₁ transgenic plants M209-1–209-10 were tested by goat anti-IgM Fc µ-chain conjugated to HRP. protein marker (M); +, tobacco plant EpCAM–IgM Fc as a positive control; − non-transgenic plant as a negative control. (B) F₁ transgenic plants M209-1–209-10 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. 2–9, T₁ transgenic plant #2–9 (Figure 6); +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant (NT) as a negative control; hygromycin phosphotransferase (HTP).