Figure 2.

CRISPR-Cas9 targeting tat inhibited acute HIV-1 escape. (a) As shown by the timeline at the top, T cells were infected with HIV-1_NL4-3 equivalent to 200 pg of p24 and cultured for 16 days, and supernatants were collected every four days for p24 measurement. To measure HIV-1-induced cytopathic effects, viable cells were counted with trypan blue exclusion every four days. The kinetics of supernatant p24 are shown by the red line (left y-axis) and cell density by the blue line (right y-axis). Assays were performed three times with average values (±SE) shown here. (b) Cellular DNA was extracted at 16 dpi and ~3 kb amplicons between tat exon 1 and rev exon 2 were amplified for each cell group. Amplicons were analyzed using gel electrophoresis, and a representative gel image is shown.