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. 2020 Nov 2;12(11):1250. doi: 10.3390/v12111250

Figure 3.

Figure 3

Trans-complementation of miRNA-deficient BFV with miR-BF2-5p restores BFV titers in bovine MICL cells. Bar diagrams of the virus titers (FFU/mL) and the number of GFP-positive focus forming units from three independent experiments. (A) MICL cells were infected first with serially diluted miRNA cassette deletion mutants (BFV-Riems 34-ΔmiRNA and BFV-MDBK 24-ΔmiRNA). Six h after infection, the corresponding miR-BF2-5p mimic and negative control were transfected. (B) MICL cells were transfected first with the miR-BF2-5p mimic and negative control. Six h after transfection, the cell were infected with serially diluted miRNA cassette deletion mutants (BFV-Riems 34-ΔmiRNA and BFV-MDBK 24-ΔmiRNA). At 4 day p.i., viral titers were determined in both experiments by fluorescence microscopy. Error bars for the titers determined are given and differences between titers of the viruses trans-complemented with the BF2-5p mimic and control miRNA were analyzed by the Welch’s t-test (***, p < 0.001).