Figure 5.

Binding efficacy of miR-BF2-5p and ANKRD17 (A) and Bif1 (B) target sequences in BFV-infected cells. MDBK and BoMac cells (as given) were infected with high-titer BFV-MDBK24 (wt BFV) and miRNA-deficient BFV-MDBK24 (ΔmiRNA) at a MOI of 0.1 or cell culture medium was added (MOCK). Two days p.i., the reporter plasmids psiCHECK2-ANKRD17-original (A.Ori, panel A) and psiCHECK2-Bif1-original (B.Ori, panel B) were transfected into the cells. At 24 h post-transfection, cell lysates were harvested and analyzed for the suppression of Rluc activity in standard DLR assays as described. Normalized luciferase data (Renilla versus firefly luciferase activity) are presented as bar diagrams of three independent experiments. Differences between treated and control groups (MOCK) were analyzed by the Welch’s t-test (***, p < 0.001).