Skip to main content
. 2020 Nov 2;12(11):1250. doi: 10.3390/v12111250

Figure 6.

Figure 6

Binding efficacy of miR-BF2-5p mimic and the target sequences in the 3′UTR of bovine ANKRD17 (A) and Bif1 (B). HEK293T, MDBK, and BoMac cells were transfected with four reporter plasmid/RNA oligonucleotide combinations (letters A and B refer to the ANKRD17 and Bif1 target genes and suffixes Ori and Scr refer to original (wild-type) and scrambled (non-functional control) target sequences, respectively): A.Scr/B.Scr plus miR-BF2-5p mimic (A.Scr/B.Scr (2–5p mimic)); A.Ori/B.Ori plus miR-BF2-5p mimic (A.Ori/B.Ori (2–5p mimic)); A.Scr/B.Scr plus mimic NC (A.Scr/B.Scr (mimic NC)); A.Ori/B.Ori plus mimic NC (A.Ori/B.Ori (mimic NC)). In addition, the empty DLR report plasmid was used as control (black bars). At 24 h post transfection, cell lysates were harvested and analyzed for the suppression of Rluc activity in standard DLR assays as described. Normalized luciferase data (Renilla versus firefly luciferase activity) are presented as bar diagrams of three independent experiments. Differences between treated groups and MOCK or Control groups were analyzed by the Welch’s t-test (***, p < 0.001; **, p < 0.01).