Figure 10.

Kinetics of ANKRD17 and Bif1 steady state levels in BFV-infected and mock-infected BoMac (lanes 1 to 8) and MDBK cells (lanes 9 to 16). Sub-confluent BoMac and MDBK cells were either mock-infected (lanes 1 to 4 and 9 to 12) or infected with wt BFV-MDBK24 at a MOI of approximately 0.1 (lanes 5 to 8 and 13 to 16) and harvested 1, 2, 3, and 4 p.i. (as indicated below the blots). Identical amounts of protein were loaded and β-actin expression was monitored for proper protein loading (bottom panel). In this blot, the 75kDa splice variant of bovine ANKRD17 (top panel) was detected using the antiserum provided by T. Kufer and the full-length 40 kDa Bif1 protein was detected using the commercially available antiserum (middle panel). The bands specific for the 75 kDa ANKRD17 form and the 40 kDa Bif and 42 kDa β-actin are shown. Below the blots, the steady state protein levels were determined by densitometry, normalized to β-actin levels, and expression levels are displayed relative to the 1 d mock- and BFV-infected BoMac and MDBK cell values.