Figure 5.

The depletion of LATS1/2 inhibits EC permeability. (A–D) Thb (2.5 U/mL)-mediated reduction in TEER values observed in HAECs treated with siCTRL (blue, A–D) was inhibited in cells treated with siLATS1 (A,B) or siLATS2 (C,D), as assessed by ECIS system and shown as normalized resistance measured approximately every 4 min for indicated times. The dashed line indicates addition of Thb. A reduction in TEER indicates an increase in cell barrier permeability (33) through paracellular mechanisms (34). (B,D) Graph demonstrates normalized resistance after Thb treatment at indicated times, relative to basal level (mean ± SEM, n = 3–4). Statistical significance was assessed using ANOVA followed by Bonferroni post-hoc testing for multiple group comparison. ***P < 0.001 and **P < 0.01. (E) Graph demonstrates that Thb (10 U/mL)-induced permeability in HUVECs treated with siCTRL, represented by fluorescence intensity measured in arbitrary units (a.u.), is further increased in HUVECs treated with siYAP (100 nM, 48 h). An increase in fluorescence intensity indicates an increase in cell barrier permeability (35, 36). Statistical significance was assessed using ANOVA followed by Bonferroni post-hoc testing for multiple group comparison. *P < 0.05.