FIGURE 3.

Expression patterns of LhFT and flowering-related genes in ‘Lollypop’ bulbs during chilling and planting. (A) Semiquantitative RT-PCR analysis of LhFT, LhFD, LhMADS5, and LhACT genes using scales (A) and SAMs (B) of ‘Lollypop’ blubs. Scale and SAM samples were collected from bulbs exposed to chilling for 0, 4, 8, 12, and 16 weeks, and from bulbs grown for 1, 2, and 3 weeks after chilling for 16 weeks. The gene names and number of cycles are indicated to the left of the panel. The amplified fragment lengths are indicated to the right of the panels. The 516-bp fragment of LhFT6 corresponds to the normal transcript, whereas the 700-bp fragment corresponds to the alternative transcript. The 540-bp fragment of LhFT8 corresponds to normal transcript, whereas the 630-bp, 725-bp, and 820-bp fragments correspond to alternative transcripts.