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. 2020 Nov 9;11:570915. doi: 10.3389/fpls.2020.570915

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Copyright © 2020 Kurokawa, Kobayashi, Nemoto, Nozawa, Sawasaki, Nakatsuka and Yamagishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temporal expression patterns of LhFT and flowering-related genes in different organs of ‘Lollypop.’ The quantitative gene expression analysis of LhFT1, LhFT4, LhFT6, LhFT8, LhFD, and LhMADS5 in SAM, scales, leaf samples of Asiatic hybrid ‘Lollypop’ was performed. Bulb scales samples were collected from bulbs under different development phase, including non-vernalization (NV) and vernalization (= 0 day after planting), 26, and 34 days after planting. Leaf samples were collected from 26, 34, 40, and 47 days after planting. SAM samples were collected from non-vernalized bulbs and vernalized bulb (0 day) and from shoots at 26, 34, 40, and 47 days after planting. Blue and orange fill boxes indicated chilling exposure period for 4 months and floral initiation period, respectively. The expression levels of LhFT and flowering-related genes were investigated by RT-qPCR and normalized using that of LhACT. Values are expressed as the mean ± SE (n = 6). Temporal expression pattern per each organ is shown in Supplementary Figure 5.