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. 2020 Oct 31;9(11):2385. doi: 10.3390/cells9112385

Figure 4.

Figure 4

Matrigel plug assays were performed to assess angiogenesis. (A) Representative images show migrated endothelial cells (arrow) that were stained for the endothelial cell marker CD31 (green) and with DAPI (blue). Matrigel plugs containing VEGF (19.1 ng/mL), SHED-CM, the <6 kDa fraction, >6 kDa fraction, SHED, or DMEM were injected subcutaneously into male C57BL/6J mice (n = 7). Scale bar = 100 μm. (B) Numbers of endothelial cells that migrated into the Matrigel plug. * p < 0.05 vs. DMEM, ** p < 0.01 vs. DMEM.