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Aortic ring assays were performed to assess the effect of SHED-CM on angiogenesis. (A) Representative images show outgrowth of neovessels from the aortic ring of a male Sprague-Dawley rat. The aortic ring was embedded in Matrigel and incubated for 14 days with VEGF (19.1 ng/mL), SHED-CM, the <6 kDa fraction, >6 kDa fraction, or DMEM (n = 3). Scale bar = 500 μm. (B) Total lengths of neovessels. * p < 0.01 vs. DMEM.
