Figure 1.
OGG1 inhibition with TH5487 results in accumulation of genomic 8-oxoG base lesions. (a) Scheme of TH5487 treatment to study the repair kinetics of genomic 8-oxoG. (b) Fluorescence signal intensity of 8-oxoG at the indicated time points. U2OS cells were pre-challenged with 20 mM KBrO3 for 1 h, then allowed to recover in a medium containing either 0.1% DMSO or 10 μM TH5487 for the indicated time points. Scatterplot shows data of at least 500 cells for each treatment condition with the mean from three independent experiments. Statistical significance was determined using an unpaired two-sided t-test (** P < 0.01, **** P < 0.0001). (c) Representative confocal images of U2OS cells showing accumulation of genomic 8-oxoG lesions. Scale bar: 50 μm.