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. 2020 Oct 26;10(11):1483. doi: 10.3390/biom10111483

Figure 4.

Figure 4

TH5487 treatment reduces OGG1 recruitment to laser-induced DNA damage sites. (a) Representative images of DMSO- and TH5487-treated U2OS cells expressing OGG1-GFP. Arrows indicate the point of laser irradiation. Scale bar: 10 μm. (b) OGG1 recruitment kinetics. U2OS cells expressing OGG1-GFP were pre-sensitized with Hoechst and treated with either 0.1% DMSO or 10 μM TH5487 for 1 h, then DNA damage was induced with a 405 nm laser. RFU (relative fluorescence units). (c) Quantification of the maximum fluorescence intensity upon OGG1-GFP recruitment. (d) Quantification of the time needed to reach maximum fluorescence of OGG1-GFP. The scatterplots show the data of at least 24 cells for each treatment condition. Data are means ± SEM (b) or means ± SD (c,d) of two independent experiments. Statistical significance was determined using unpaired two-sided t-tests (** P < 0.01, *** P < 0.001).