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. 2020 Nov 2;12(11):1249. doi: 10.3390/v12111249

Table 1.

Summary of samples used from in vivo and in vitro experiments. NDV, Newcastle disease virus; CEF, chicken embryo fibroblast.

Melissa Deist & Lamont Lab Group [21,22,23] Prof Chan Ding’s Lab [20]
Samples Chicken Line Sex Phenotype Chicken Age Samples Embryo Age
Leghorn rep 1 Leghorn Female Susceptible 21 LaSota rep 1 CEF cells isolated from 10-day-old SPF chicken embryos
Leghorn rep 2 Leghorn Female Susceptible 21 LaSota rep 2
Leghorn rep 3 Leghorn Male Susceptible 21 LaSota rep 3
Fayoumi rep 1 Fayoumi Female Resistant 21 Herts/33 rep 1
Fayoumi rep 2 Fayoumi Female Resistant 21 Herts/33 rep 2
Fayoumi rep 3 Fayoumi Male Resistant 21 Herts/33 rep 3
Virus dose 200 microliters 107 embryo infectious dose of 50% Virus dose MOI = 1
Experiment type in vivo Experiment type in vitro
Organ harvested Trachea Organ used for primary cells Chicken embryo
Cell type Epithelial cell Cell type Fibroblast cell
Sample type RNA Sample Type RNA
Time of tissue harvest 2 days post infection Time of cell harvest 12 h post infection
NDV strain LaSota (non-pathogenic) NDV strain LaSota
(non-pathogenic)
& Herts/33
(highly pathogenic)