Table 1.
Melissa Deist & Lamont Lab Group [21,22,23] | Prof Chan Ding’s Lab [20] | |||||
---|---|---|---|---|---|---|
Samples | Chicken Line | Sex | Phenotype | Chicken Age | Samples | Embryo Age |
Leghorn rep 1 | Leghorn | Female | Susceptible | 21 | LaSota rep 1 | CEF cells isolated from 10-day-old SPF chicken embryos |
Leghorn rep 2 | Leghorn | Female | Susceptible | 21 | LaSota rep 2 | |
Leghorn rep 3 | Leghorn | Male | Susceptible | 21 | LaSota rep 3 | |
Fayoumi rep 1 | Fayoumi | Female | Resistant | 21 | Herts/33 rep 1 | |
Fayoumi rep 2 | Fayoumi | Female | Resistant | 21 | Herts/33 rep 2 | |
Fayoumi rep 3 | Fayoumi | Male | Resistant | 21 | Herts/33 rep 3 | |
Virus dose | 200 microliters 107 embryo infectious dose of 50% | Virus dose | MOI = 1 | |||
Experiment type | in vivo | Experiment type | in vitro | |||
Organ harvested | Trachea | Organ used for primary cells | Chicken embryo | |||
Cell type | Epithelial cell | Cell type | Fibroblast cell | |||
Sample type | RNA | Sample Type | RNA | |||
Time of tissue harvest | 2 days post infection | Time of cell harvest | 12 h post infection | |||
NDV strain | LaSota (non-pathogenic) | NDV strain | LaSota (non-pathogenic) & Herts/33 (highly pathogenic) |