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. 2020 Oct 26;9(11):1442. doi: 10.3390/plants9111442
BLAST Basic local alignment search tool
CLD Control cold treatment (7–9 days at 4 °C)
MAN Control mannitol treatment (0.7 M mannitol at 25 °C for 5 days)
C0T Cold treatment with 1% DMSO
C0.1T Cold treatment with 0.1 µM TSA
C0.2T Cold treatment with 0.2 µM TSA
C0.4T Cold treatment with 0.4 µM TSA
C + 0T Cold treatment plus 2 h with 1% DMSO
C + 0.1T Cold treatment plus 2 h with 0.1 µM TSA
C + 0.2T Cold treatment plus 2 h with 0.2 µM TSA
C + 0.4T Cold treatment plus 2 h with 0.4 µM TSA
DAPI 4′,6-Diamidine-2’-phenylindole dihydrochloridl
DH Doubled haploid
EMB Number of embryos/100 anthers
FM Fresh microspores
GP Number of green plants/100 anthers
GPDH Number of green DH plants/100 anthers
ME Microspore embryogenesis
M0T Mannitol treatment with 1% DMSO
M0.1T Mannitol treatment with 0.1 µM TSA
M0.2T Mannitol treatment with 0.2 µM TSA
M0.4T Mannitol treatment with 0.4 µM TSA
M + 0T Mannitol treatment plus 2 h with 1% DMSO
M + 0.1T Mannitol treatment plus 2 h with 0.1 µM TSA
M + 0.2T Mannitol treatment plus 2 h with 0.2 µM TSA
M + 0.4T Mannitol treatment plus 2 h with 0.4 µM TSA
PDH Percentage of DH plants/total green plants analyzed
PEMB Number of proembryos /100 anthers
PGP Number of green plants/total plants
RNA Ribonucleic acid
RT Room temperature
SLM “Star-like” morphology
TSA Trichostatin A
0T 1% DMSO treatment for 2 h
0.1T 0.1 µM TSA treatment for 2 h
0.2T 0.2 µM TSA treatment for 2 h
0.4T 0.4 µM TSA treatment for 2 h