BLAST |
Basic local alignment search tool |
CLD |
Control cold treatment (7–9 days at 4 °C) |
MAN |
Control mannitol treatment (0.7 M mannitol at 25 °C for 5 days) |
C0T |
Cold treatment with 1% DMSO |
C0.1T |
Cold treatment with 0.1 µM TSA |
C0.2T |
Cold treatment with 0.2 µM TSA |
C0.4T |
Cold treatment with 0.4 µM TSA |
C + 0T |
Cold treatment plus 2 h with 1% DMSO |
C + 0.1T |
Cold treatment plus 2 h with 0.1 µM TSA |
C + 0.2T |
Cold treatment plus 2 h with 0.2 µM TSA |
C + 0.4T |
Cold treatment plus 2 h with 0.4 µM TSA |
DAPI |
4′,6-Diamidine-2’-phenylindole dihydrochloridl |
DH |
Doubled haploid |
EMB |
Number of embryos/100 anthers |
FM |
Fresh microspores |
GP |
Number of green plants/100 anthers |
GPDH |
Number of green DH plants/100 anthers |
ME |
Microspore embryogenesis |
M0T |
Mannitol treatment with 1% DMSO |
M0.1T |
Mannitol treatment with 0.1 µM TSA |
M0.2T |
Mannitol treatment with 0.2 µM TSA |
M0.4T |
Mannitol treatment with 0.4 µM TSA |
M + 0T |
Mannitol treatment plus 2 h with 1% DMSO |
M + 0.1T |
Mannitol treatment plus 2 h with 0.1 µM TSA |
M + 0.2T |
Mannitol treatment plus 2 h with 0.2 µM TSA |
M + 0.4T |
Mannitol treatment plus 2 h with 0.4 µM TSA |
PDH |
Percentage of DH plants/total green plants analyzed |
PEMB |
Number of proembryos /100 anthers |
PGP |
Number of green plants/total plants |
RNA |
Ribonucleic acid |
RT |
Room temperature |
SLM |
“Star-like” morphology |
TSA |
Trichostatin A |
0T |
1% DMSO treatment for 2 h |
0.1T |
0.1 µM TSA treatment for 2 h |
0.2T |
0.2 µM TSA treatment for 2 h |
0.4T |
0.4 µM TSA treatment for 2 h |