Table 2.
Microspore embryogenesis of bread wheat cvs. Pavon and Caramba after the application of 0, 0.1, 0.2, and 0.4 µM TSA simultaneously with mannitol stress treatment for 5 days at 25 °C (M0T, M0.1T, M0.2T, and M0.4T) and MAN (control culture, 0.7M mannitol for 5 days at 25 °C). Table shows TSA treatments means after statistical analysis of number of proembryos (PEMB), embryos (EMB), green plants (GP), and green DH plants (GPDH), all per 100 anthers, and percentages of green plants (PGP, number of green plants/total plants) and spontaneous chromosome doubling (PDH, percentages of DH plants/total green plants analyzed).
| Cultivar | PEMB * | EMB * | GP * | PGP (%) | PDH (%) | GPDH * |
|---|---|---|---|---|---|---|
| Pavon | ||||||
| MAN | 211.7 b† | 59.2 b | 25.9 b | 73.7 a | 28.0 b | 7.2 b |
| M0T | 185.8 b | 70.7 ab | 33.1 ab | 66.3 a | 52.7 a | 17.8 ab |
| M0.1T | 402.9 ab | 113.4 a | 43.8 ab | 75.9 a | 56.2 a | 24.6 ab |
| M0.2T | 563.0 a | 115.8 a | 52.3 ab | 75.5 a | 44.0 ab | 23.0 ab |
| M0.4T | 516.1 a | 120.3 a | 52.1 a | 71.0 a | 58.9 a | 30.7 a |
| Caramba | ||||||
| MAN | 162.2 c | 30.4 c | 5.1 b | 49.4 a | 53.2 b | 2.7 b |
| M0T | 287.1 bc | 55.7 bc | 12.0 ab | 67.7 a | 56.5 b | 6.8 ab |
| M0.1T | 499.5 ab | 78.9 ab | 13.5 ab | 59.4 a | 60.0 b | 8.1 ab |
| M0.2T | 564.0 ab | 74.9 ab | 11.4 ab | 45.0 a | 79.3 a | 9.0 ab |
| M0.4T | 890.3 a | 97.7 a | 18.2 a | 69.5 a | 72.6 ab | 13.2 a |
* Values based on 100 anthers. † Values followed by the same letter within each cultivar and variable are not significantly different (p ˂ 0.05), according to a Duncan or a Chi square test.