Table 1.
List of antibodies used in the study.
| Antibody | Target | Company | Cat.No | Dilution IF | Dilution IB |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| MJFR-1 | human alpha-synuclein | Abcam | ab138501 | 1: 1000 | 1: 10,000 |
| EP1536Y | pS129 phospho-synuclein | Abcam | ab51253 | 1: 500 | 1: 5000 |
| Syn1 clone 42 | human and murine alpha-synuclein | BD Biosciences | 610787 | 1: 500 | 1: 2000 |
| D37A6 | murine alpha-synuclein | Cell Signaling | #4179 | 1: 200 | 1: 2000 |
| SynF1 | aggregated alpha-synuclein | BioLegend | 847802 | 1: 500 | 1: 10,000 |
| syn211 | human alpha-synuclein | Abcam | ab80627 | 1: 500 | n/a |
| MBP | myelin basic protein | Abcam | ab218011 | 1: 200 | 1: 2000 |
| CNPase | cyclic nucleotide phospho. | Abcam | ab6319 | 1: 500 | n/a |
| Sox10 | sox10 protein | Abcam | ab155279 | 1: 200 | n/a |
| NeuN | neuronal nuclei protein | Merck Millipore | MAB377 | 1: 500 | n/a |
| Actin | beta-actin | Sigma | A5316 | n/a | 1: 10,000 |
| Secondary antibodies | |||||
| Goat anti-mouse HRP | mouse IgG (H + L) | Jackson Immuno | 115-035-146 | n/a | 1: 10,000 |
| Goat anti-rabbit HRP | rabbit IgG (H + L) | Jackson Immuno | 111-035-144 | n/a | 1: 10,000 |
| Goat anti-mouse IRDye 680RD | mouse IgG (H + L) | LI-COR | 926-68070 | n/a | 1: 5000 |
| Goat anti-rabbit IRDye 800CW | rabbit IgG (H + L) | LI-COR | 926-32211 | n/a | 1: 5000 |
| Donkey anti-mouse Alexa 488 | mouse IgG (H + L) | Thermo Fisher Sci. | A-21202 | 1: 500 | n/a |
| Goat anti-rabbit Alexa 488 | rabbit IgG (H + L) | Thermo Fisher Sci. | A-11008 | 1: 500 | n/a |
| Donkey anti-mouse Alexa 594 | mouse IgG (H + L) | Thermo Fisher Sci. | A-21203 | 1: 500 | n/a |
| Donkey anti-rabbit Alexa 594 | rabbit IgG (H + L) | Thermo Fisher Sci. | A-21207 | 1: 500 | n/a |
IF: Immunofluorescence; IB: Immunoblotting.