Table 1.
Logarithmic reduction of African swine fever virus (ASFV) titer in the presence of tested active substances. Contact time, 30 min. Temperature of incubation, 10 °C. Each data point is the mean of three experiments, ±SD.
| Active Substance | Tested Concentration | Log10 Difference ** (±SD) (TCID50/mL) | Virucidal Effect (Difference ≥ 4 Log10) | ||
|---|---|---|---|---|---|
| BSA | BSA + YE | BSA | BSA + YE | ||
| Sodium Hypochlorite | 1.5% | 5.3 (±0.35) | 4.58 (±0.47) | Yes | Yes |
| 1% | 5.3 (±0.35) | 5.58 (±0.47) | Yes | Yes | |
| 0.3% | 5.3 (±0.35) | 4.17 (±0.31) | Yes | No | |
| Caustic Soda | 3% | 4.67 * (±0.77) | 4.67 * (±0.51) | N/A | Yes |
| 2% | 4.91 (±0.59) | 5.17 (±0.24) | Yes | Yes | |
| 1% | 4.83 (±0.67) | 4.17 (±0.31) | Yes | No | |
| Phenol | 2% | 3.42 * (±0.24) | 3.75 * (±0.20) | N/A | N/A |
| 1% | 4.42 (±0.24) | 4.75 (±0.20) | Yes | Yes | |
| 0.5% | 0.08 (±0.12) | 0.08 (±0.12) | No | No | |
| Potassium Peroxymonosulfate | 2% | 3.75 *(±0.20) | 4.17 *(±0.24) | N/A | N/A |
| 1% | 4.75 (±0.20) | 5.17 (±0.24) | Yes | Yes | |
| 0.5% | 4.75 (±0.20) | 5.17 (±0.24) | Yes | Yes | |
| Acetic Acid c | 3% | 4.33 (±0.23) | 5 (±0.2) | Yes | Yes |
| 2% | 4.33 (±0.23) | 3 (±0.2) | Yes | No | |
| 1% | 3.92 (±0.12) | 1.42 (±0.12) | N/A | No | |
| Glutaraldehyde c | 1% | 4.33 (±0.31) | 4 * (±0) | Yes | Yes |
| 0.5% | 4.33 (±0.31) | 4 * (±0) | Yes | Yes | |
| 0.1% | 4.33 (±0.31) | 5 (±0) | Yes | Yes | |
| Formaldehyde c | 1.6% | 2.08 * (±0.31) | 1.67 * (±0.24) | N/A | N/A |
| 0.8% | 1.83 * (±0.31) | 2.5 * (±0.0) | N/A | N/A | |
| 0.4% | 2.83 *(±0.31) | 2.67 * (±0.24) | N/A | N/A | |
| Benzalkonium Chloride c | 2% | 2.25 * (±0) | 2.67 * (±0.12) | N/A | N/A |
| 1% | 4.25 (±0) | 3.75 * (±0) | Yes | N/A | |
| 0,5% | 4.08 (±0.11) | 3.75 * (±0) | No | N/A | |
** Difference between control and tested sample. * Cytotoxic effect. N/A = the virucidal effect could not be evaluated, due to cytotoxicity. BSA = low soiling level (bovine serum albumin 3.0 g/L), BSA + YE; c cytotoxic effect was observable—results are presented after applying the cytotoxicity neutralization.