Table 1.

Liquid biopsies and EV EGFR.

Cancer	EV Type	Biomolecule	EGFR Biomarker	EV Source	Isolation/Detection Method	Ref.
Breast	Small EVs	Protein	WT	Plasma	DC; Immunosensor	[31]
Glioblastoma	EVs *	RNA	WT, EGFRvIII	CSF	DC; PCR	[32]
Glioblastoma	Small EVs	RNA	WT, EGFRvIII	Plasma	Total Exosome Isolation Kit; PCR	[33]
Glioblastoma	Small EVs	Protein	WT	Serum	DC; Flow cytometry	[34]
Glioblastoma	Small EVs	Protein	WT, EGFRvIII	Serum	DC; Electrochemical biosensor	[35]
Lung	Small EVs	Protein	WT	Plasma	Dielectrophoretic chip; Immunofluorescence	[36]
Lung	EVs	RNA	T790 M	Plasma	NanoVilli Chip; PCR	[37]
Lung	EVs *	DNA/RNA	Ex19 del, T790 M	Plasma	ExoQuick; PCR	[38]
Lung	EVs *	DNA	WT, EGFR mutation panel	Pleural Effusions	ExoQuick; PCR	[39]
Lung	EVs *	DNA/RNA	WT, EGFR mutation panel	Plasma	Exolution; PCR	[40]
Lung	Medium EVs	DNA	WT, EGFR mutation panel	BALF	DC; PCR	[41]
Lung	Medium EVs	DNA	WT, EGFR mutation panel	Pleural Effusions	DC; PCR	[42]
Ovarian	Small EVs	Protein	WT	Plasma	DC; Microfluidic device	[43]
Prostate	Small EVs	Protein	WT	Plasma	DC; ELISA	[44]

Abbreviations: Small EVs = EVs <200 nm, Medium EVs = EVs <500 nm, EVs = EVs <1000 nm, WT = wild-type, CSF = Cerebrospinal fluid, BALF = Bronchoalveolar lavage fluid, DC = Differential centrifugation, T790M = threonine to methionine missense substitution at position 790 in exon 20 of EGFR, Ex19 del = in-frame deletion in exon 19 of EGFR. * EV characterisation not performed to MISEV2018 guidelines; no information on EV size range.