Figure 4.

6OHDA triggered apoptotic death in dDCNs. To determine if 6OHDA stimulated death of dDCN is via the apoptotic or necrotic route, the TUNEL assay was used. Various inhibitors such as IKK, zVADfmk, zVDVADfmk, and zIETDfmk were used with 6OHDA to determine if the inhibitor decreased 6OHDA-mediated death of dDCNs. 6OHDA triggered death of dDCNs via the apoptotic route. The result illustrates that all studied inhibitors reduced apoptotic death of dDCNs at various rates. The result is shown as the relative percentage of cell death compared with control. The (+) and (-) signs indicate with or without treatment respectively. Means of three experiments ± SEM are shown. A table of values and statistical analysis can be found in supplementary data S6.