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Expression analysis of candidate genes in roots of alfalfa. Relative expression of MsIRT1 (a), MsSultr1;2 (b), MsSultr1;3 (c), MsPCS1 (d) and MsGSH1(e) in alfalfa cultivated in different conditions of Hg and GSH: +Hg (40 μM), +Hg+GSH (40 μM Hg+50 μM GSH), and +GSH (50 μM GSH). Data represent means ± SD of three independent biological samples. Different letters indicate a significant difference at the p < 0.05 level.
