Effect of prebiotics on the growth of B. infantis IM-1® after 24 h of incubation. (A) Optical density (OD660 nm) of the cultures of the B. infantis IM-1® strain grown in the presence of different prebiotic substrates. The represented data are means of at least three independent replicates. (B) Statistically significant differences in growth of B. infantis IM-1® in the presence of different prebiotics were determined, using Student’s t-test for each pair of substrates tested. Color key: dark blue: p > 0.05; light blue: * p < 0.05; light red: ** p < 0.01; and dark red: *** p. < 0.001. FOS, fructooligosaccharides; GOS, galactooligosaccharides; Fru; Frutalose, and control (MRSFc: Man, Rogosa and Sharpe broth with 0.05% of L-cysteine hydrochloride monohydrate. MRSFc without prebiotic supplementation).