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. 2020 Oct 30;11(11):1296. doi: 10.3390/genes11111296

Figure 3.

Figure 3

Phylogenetic analysis of characterised siderophore transporters from S. cerevisiae (Sc, brown), S. pombe (Sp, pink), C. albicans (Ca, blue), C. neoformans (Cn, red), A. fumigatus (Af, torquoise), and A. nidulans (An, green). Putative siderophore transporters from L. corymbifera (Lc, purple) are also included. CnTri12 is a major facilitator not belonging to the SIT-family of proteins and serves as an outgroup. All sequences were aligned using MUSCLE (v.3.8.31, Marceille, France). Phylogenetic tree was reconstructed using the maximum likelihood method implemented in the PhyML program (v3.1/3/0 aLRT, Marceille, France). The WAG substitution model was selected assuming an estimated proportion of invariant sites (0.011) and 4 gamma-distribution rate categories to account for rate heterogeneity across sites. The gamma shape parameters were estimated directly from the data (gamma = 2.830). Reliability for internal branch was assessed using the aLRT test (SH-Like) [127,128,129,130,131,132,133].