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Effect of different passages on D-17 cells gene expression. Data are expressed as 2-ΔΔCq; values are the mean of three test replicates and ΔΔCq = ΔCq (305th or 262th passage)—ΔCq (control 255th passage). Negative samples were given a Cq 39 fictitious value. Data were checked by ANOVA for different value of significance (p < 0.05; p < 0.01; p < 0.001; p < 0.0001).
