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. 2020 Oct 28;10(11):1981. doi: 10.3390/ani10111981

Table 1.

Characteristics of the primers used and the bibliographic references.

Gene Primers Efficiency R2 Slope References/
Designed
RPS5 F: TCACTGGTGAGAACCCCCT
R: CCTGATTCACACGGCGTAG
106.9% 0.998 −3.167 [9]
B2M F: TCCTCATCCTCCTCGCT
R: TTCTCTGCTGGGTGTCG
111.8% 0.990 −3.000 [9]
GUSB F: AGACGTTCCAAGTACCCC
R: AGGTGTGGTGTAGAGGAGCAC
99.7% 0.998 −3.328 [9]
GAPDH F: CTGGGGCTCACTTGAAAGG
R: GGAGGCATTGCTGACAATC
96.7% 0.999 −3.404 Designed
HPRT1 F: CTGAAGAGCTACTGTAATGACCAGTC
R: CTTTTCACCAGCAAGCTTGCAACC
101.6% 0.987 −3.284 Designed
RPL13A F: GGGGCAGGTCCTGGTGCTCG
R: CCAGGTACTTCAACTTGTTTCTGTAG
97.7% 0.999 −3.378 Designed
RPS19 F: CCTTCCTCAAAAAGTCTGGG
R: GCTGTGGAAGCAGCTCGC
97.0% 0.999 −3.396 [10]
Designed
SDHA F: GGTGGCACTTCTACGACACC
R: CCATAATTCTCCAGCTCTACC
103.7% 1.000 −3.237 [11]
Designed