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IgA antibodies to subtype C (CN54) gp140. IgG-depleted serum samples were used to assess induction of monomeric IgA binding to subtype C HIV-1 Env. Depletion of IgG reactivity was confirmed using anti-Env IgG ELISA (A). IgA antibodies were significantly more frequent in recipients of a combination of HIV-MVA and adjuvanted CN54rgp140 than in HIV-MVA vaccinees, p = 0.0031 (B). The dotted line indicates the first dilution used.
