Figure 7.

Magnitude of ADCC-mediating antibody responses in responding vaccinees. ADCC-mediating antibody responses to CM235 CRF01_AE infected cells were assessed using luciferase assay. No significant difference in titers of ADCC responses was seen among responding vaccinees in the two groups. Polyclonal purified IgG from HIV infected donors was used as positive control while serum from an HIV-uninfected individual was used as negative control. The titer of ADCC-mediating antibodies was defined as the reciprocal of highest dilution showing more than 15% specific killing activity (determined based on the responses seen prior to immunization to allow for 2% false positive rate) after background subtraction. The dotted line indicates the first dilution used.