Figure 1.

Growth phenotypes of mutant plants devoid of Trxs y. (A) Wild-type (WT) and mutant lines, as indicated, were grown under short-day conditions for 6 weeks. (B) Western blot analysis of the content of NTRC and Trx y2. Protein extracts obtained from leaves of plants grown as stated in (A), were subjected to SDS–PAGE under reducing conditions, transferred to nitrocellulose filters and probed with anti-NTRC or anti-Trx y2 antibodies. Even loading was monitored by Ponceau staining of the Rubisco large subunit and molecular weight markers (kDa) are indicated on the left. The fresh weight (C) and the content of chlorophylls (D) of rosette leaves from fourteen plants of each line are represented as average values ± standard error (SE). Statistical significance compared with the wild type (asterisks) or the ntrc mutant (hashes) is indicated (*** and ###, P < 0.001, Student’s t test). Letters indicate significant differences (P < 0.05) determined by one-way ANOVA followed by Tukey’s post-test.