Figure 4.

Fluorescent immunohistochemical labeling of BrdU (red) and fluorescent detection of EdU (green) after injection of different doses of the proliferation markers. Images show VZ_TSM after 10, 50, and 225 mg/kg BrdU or equimolar doses of EdU. BrdU was detected by the antibody OBT0030. The dose 50 mg/kg of BrdU resulted in more labeled cells than the dose 10 mg/kg but did not differ from the dose 225 mg/kg. The same applies for equimolar doses of EdU. Medial is left, lateral is right. The white scale bar in the last image represents 100 μm.